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The Twig IVF Roadmap: A Step-by-Step Guide to Your In Vitro Fertilization Journey

Dr. Rhonda Zwingerman, MD MSc FRCSC (GREI) and Michelle Moniz, RN, MN, BScN3 min readJan 2026
Cover image for The Twig IVF Roadmap: A Step-by-Step Guide to Your In Vitro Fertilization Journey

Starting an In Vitro Fertilization (IVF) cycle is a significant step in your family-building journey.

Starting an In Vitro Fertilization (IVF) cycle is a significant step in your family-building journey. IVF is an assisted reproduction method where eggs and sperm are combined to create embryos in a lab.

Here is a supportive, step-by-step guide to the IVF roadmap at Twig:

Step 1: Preparation & Priming

This step happens in the months before your cycle starts.

  • Personalized Plan: You’ll have an appointment with your fertility specialist to plan and review your personalized treatment plan.

  • Priming Medications: Your physician may ask you to start medication to help prepare your ovaries for the upcoming stimulation. However your physician will discuss with you whether these medications are recommended.

Step 2: Contact Us on Your Day 1

When your period begins, you’ll report your Day 1 (the first day of full flow) to officially start your treatment cycle.

Step 3: Ovarian Stimulation & Cycle Monitoring

This phase typically lasts 8 to 13 days and involves stimulating your ovaries to produce multiple eggs.

  • Injections: You will take daily injectable hormonal medications to stimulate your ovaries. You may be instructed to add a second medication a few days later to prevent early ovulation. Your nurse will teach you how to use them during your first visit.

  • Monitoring: We will closely track your progress with blood work and internal ultrasounds (cycle monitoring) every few days. Most people require 4-5 visits during the course of ovarian stimulation.

  • The Trigger: Once your follicles are ready, you will take trigger medications on a specific date and time to help them mature in preparation for the retrieval.

Step 4: Egg Retrieval

Exactly 36 hours after your trigger injections, one of our physicians will carefully retrieve your eggs.

  • Procedure: We use an ultrasound to guide a needle gently through the top of the vagina into the ovaries. We use conscious sedation to ensure you are comfortable.

  • Lab Hand-off: The retrieved eggs are immediately handed off to our in-house embryology lab to be counted and prepared for fertilization.

Step 5: Fertilization & Embryo Culture

Once retrieved, your eggs are immediately handed off to our embryologist to be assessed and frozen.

  • Fertilization: A few hours after retrieval, the embryologist fertilizes the eggs with sperm, either through conventional IVF or ICSI.

  • Culture: Over the next 5 to 7 days, the embryos are kept safe and monitored closely in our time-lapse incubator to track their development.

  • Updates: You will receive regular updates from our embryology team about your embryos’ progress.

Step 6: Embryo Freezing, Transfer or Biopsy

Depending on your treatment plan, your next step will be one of the following:

Option A: Embryo Freezing Option B: Embryo Transfer Option C: Embryo Biopsy & Testing Freezing: Any fully developed embryos are frozen for future use. Fresh Transfer: Five days after the egg retrieval, an embryo is placed into the uterus under ultrasound guidance by one of our physicians.

Freezing: Any fully developed embryos that are not transferred are frozen for future use. Preimplantation Genetic Testing (PGT): If your treatment plan includes PGT, any fully developed embryos will be carefully biopsied before being frozen.

Results: Once results are ready, they are reviewed with you by one of Twig’s in-house genetic counsellors.

Ready to learn more? Click here to book a complimentary family building consult. No referral required.

Written by

Dr. Rhonda Zwingerman
MD MSc FRCSC (GREI)
Michelle Moniz
RN, MN, BScN

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